26S 蛋白酶体
(无标签,人类)
通过无标签分级法从人类血红细胞中纯化
50微克-160美金
产品详情
我们提供的26S蛋白酶体为高度纯化产品,适用于对已经被适量泛素化的蛋白质底物进行体外的蛋白质降解研究。
来源:通过无标签分级法从人体血红细胞中纯化。表现出最高酶活性,可溶且均匀的。我们提供的的26蛋白酶体产品,由两种蛋白复合体组成:被19S调节亚基复合体所单帽(26S)和双帽(30S)覆盖其20S核心颗粒末端的蛋白复合体。其制作时的混合比例为40% 单帽 :60% 双帽蛋白酶体。
配方:于10mM TRIS,pH 7.0的缓冲液中,含有25mM氯化钾、1.1mM氯化镁、0.1mM乙二胺四乙酸、1mM二硫苏糖醇、1mM叠氮化钠、2mM ATP和35%甘油。
缓冲液成分可根据客户需求进行调整。
运输方式:干冰运输
长期储存:-80°C
使用与注意事项
当准备使用蛋白酶体时,请放置于冰上进行解冻。由于该26S蛋白酶体复合物的不稳定性,如果需要测量其的酶活性,请在解冻蛋白酶体后立刻使用。26S复合体解体后,20S蛋白酶体的活性相对稳定。
产品参考文献
1) UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome, Roland Hjerpe, Thimo Kurz et al.; Cell Volume 166, Issue 4, 11, 935-949, (2016), Full Text
2) Structural Snapshots of 26S Proteasome Reveal Tetraubiquitin-Induced Conformations, Zhanyu Ding, Michael H. Glickman, Yao Cong et al.; Molecular Cell Volume 73, Issue 6,1150-1161.e6, (2019), Full Text
3) Synthetic Uncleavable Ubiquitinated Proteins Dissect Proteasome Deubiquitination and Degradation, and Highlight Distinctive Fate of Tetraubiquitin Sumeet K. Singh, Ashraf Brik et al.; Journal of the American Chemical Society 138 (49), 16004-16015, (2016), Full Text
4) Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status, L. Bramasole, E. Pick et al.; Redox Biology, Elsevier, 20:533-543, (2019) Full Text
5) Tuning the proteasome to brighten the end of the journey, Thibault Mayor, Michal Sharon and Michael H. Glickman, American Journal of Physiology - Cell Physiology, 311(5):C793-C804, (2016), Full Text
6) Polyubiquitin-Photoactivatable Crosslinking Reagents for Mapping Ubiquitin Interactome Identify Rpn1 as a Proteasome Ubiquitin-Associating Subunit, Michal Chojnacki, Michael H. Glickman et al.;, Cell Chemical Biology 24(4):443-457.e6., (2017) Full Text
7) Proteasome in action: substrate degradation by the 26S proteasome: Indrajit Sahu and Michael H. Glickman, Biochem. Soc. Trans.49 (2): 629–644, (2021), Full Text
8) Reaction of complement factors and proteasomes in experimental encephalitis, Stefan Lange, Ivar Lönnroth et al.; J. Neurovirol. 23, 313-318, (2017), Full Text
9) Molecular architecture of the 26S proteasome holo complex determined by an integrative approach, Keren Lasker, Wolfgang Baumeister et al.; Proc. Natl. Acad. Sci. 31:109(5)1380-1387, (2012), Full Text