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PRODUCT DETAILS
Product specifications
The 20S proteasome is the catalytic core of the 26S proteasome, the central protease of the ubiquitin pathway of protein degradation. Unlike the 26S proteasome, which degrades proteins in an ATP-dependent manner, the 20S proteasome is ATP-independent and only degrades entirely unfolded polypeptides.
The 20S proteasome purified in our lab is free of aggregates and highly suitable for cryo-electron microscopy (cryo-EM), as shown in work by Jason E. Gestwicki and colleagues from UCSF (Full text).
SOURCE
Purified by fractionation from human erythrocytes.
Highly active, soluble, and homogeneous
FORMULATION
Liquid.
In 20mM Tris-HCl, pH 7.2, containing 1mM EDTA,
1mM 2-mercaptoethanol, and 50% glycerol.
Buffer ingredients can be adjusted to customer needs
SHIPPING
Shipped on dry ice
LONG-TERM STORAGE
Store at −80°C. Avoid repeated freeze/thaw cycles
PACKAGE
50 μg standard one unit quantity.
Contact us for larger quantities and custom requirements
UNIT PRICE
155 $ USD per 50 μg unit
PRODUCT QUALITY
Product quality assessment
(A) Coomassie-stained 12% SDS-PAGE of purified human 20S proteasome. (B) Purified human 20S proteasomes were separated by 4% native-PAGE and analyzed by overlay assays using Suc-LLVY-AMC. (C) Coomassie-stained 12% SDS-PAGE following a final polishing step, depicting untagged human standard 20S proteasome isolated by fractionation from human red blood cells.
LITERATURE
Literature references
A. Opoku-Nsiah, Jason E. Gestwicki, et al. • Nat Commun. 13(1):1226 • 2022
I. Sahu and Michael H. Glickman • Biomolecules, 11(2), 148 • 2021