PRODUCT DETAILS
Product specifications
Originally identified in Arabidopsis as a suppressor of light-dependent development and is essential for development in plants, and is itself both a target for kinase activity and associates with and coordinates activity of kinases.
Although the CSN complex and the ‘lid’ of the 19S regulator of the 26S proteasome share significant structural similarities, their functions seem to be different and neither protein-protein interactions nor associated activities described for the CSN have been identified for the 19S ‘lid’. The CSN appears to be a platform connecting signaling with degradation by the 26S proteasome.
SOURCE
Purified by fractionation from human erythrocytes.
Highly active, soluble, and homogeneous
FORMULATION
Liquid.
In 20mM Tris-HCl, pH 7.2, containing, 50mM KCl, 1mM EDTA,
1mM 2-mercaptoethanol, and 50% glycerol.
Buffer ingredients can be adjusted to customer needs
SHIPPING
Shipped on dry ice
LONG-TERM STORAGE
Store at −80°C. Avoid repeated freeze/thaw cycles
PACKAGE
50 μg standard one unit quantity.
Contact us for larger quantities and custom requirements
UNIT PRICE
300 $ USD per 50 μg unit
PRODUCT QUALITY
Product quality assessment
The CSN complex was purified to homogeneity from human blood.
(A) Coomassie-stained 12% SDS-PAGE describes the eight purified CSN subunits. (B) Immunoblotting describing Ub-like protein Nedd8, removed by CSN-mediated deneddyylation.
LITERATURE
Selected literature
A. Golan, et al. • J. Methods. Mol. Biol, 1449:103-16 • 2016
D. Dubiel, et al. • J. FEBS Lett: 2507-13 • 2015
E. Pick, A. Golan, et al. • J. PLOS ONE: 7(8):e43980 • 2012
L. Bramasole, E. Pick, et al. • Biomolecules, 9(9), 449 • 2019