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PRODUCT DETAILS
Product specifications
The eukaryotic proteasome contains 7α-type and 7 β-type subunits. Upon stimulation with IFN-γ, the three active β subunits, β1 (Y), β2 (Z), and β5 (X), are exchanged for their immunocounterparts, namely β1i (LMP-2), β2i (MECL-1), and β5i (LMP-7). This results in a change in proteasomal substrate specificity. Some groups have reported increases in chymotrypsin-like and trypsin-like activities, while others have reported no changes or even decreased activities after such treatment.
SOURCE
Purified by fractionation from human spleen tissue.
Highly active, soluble, and homogeneous
FORMULATION
Liquid.
In 20mM Tris-HCl, 1mM sodium azide, 1mM DTT, pH 7.2.
Contains 50% glycerol.
Buffer ingredients can be adjusted to customer needs
SHIPPING
Shipped on dry ice
LONG-TERM STORAGE
Store at −80°C. Avoid repeated freeze/thaw cycles
PACKAGE
50 μg standard one unit quantity.
Contact us for larger quantities and custom requirements
UNIT PRICE
350 $ USD per 50 μg unit
PRODUCT QUALITY
Product quality assessment
(A) Chromatogram depicting the separation. (B) Immunoblotting of 20S immunoproteasome (LMPT-7) and standard 20S (MB1). (C) Coomassie stained 12% SDS-PAGE following a final polishing step. A complete separation between immunoproteasome 20S (I-20S) and standard 20S (S-20S) from human spleen tissue is achieved.
C
A
B
LITERATURE
Selected literature
Conformational maps of human 20S proteasomes reveal PA28- and immuno-dependent inter-ring crosstalks
Jean Lesne and Julien Marcoux et al. • Nat Commun.,11: 6140 • 2020
Scott Ugras and Harry Ischiropoulos et al. • EBioMedicine., 31: 307–319 • 2018
Sabelle Jallow and Sarah L. Rowland‐Jones et al. • Eur J Immunol., 45(8): 2232–2242 • 2015
Andrew. M. Pickering and Kelvin. J. A. Davies • Arch Biochem Biophys., 523(2): 181–190 • 2012
Simone Cenci and Paolo Cascio et al. • J Leukoc Biol., 92(5):921-31 • 2012