Product Details

Source: Purified from human blood cells by fractination. The highest activity, soluble and homogenous.

Concentration: 1.0mg/ml
Formulation: Liquid. In 20mM TRIS-HCl, pH 7.2, containing 1mM EDTA, 1mM DTT, 1mM sodium azide and 50% glycerol.
Shipping: Shipped on Dry Ice
Long Term Storage: -80°C

Use/Stability

The Proteasome 20S purified in our lab without aggregates is ideal for Cryo-EM (Cryogenic electron microscopy), for example as conducted in the study of Jason E. Gestwicki. [ PubMed ]

20S Proteasome remains relatively stable and active so long as repetitive freeze-thaw cycles are avoided.
It has been demonstrated that 20S proteasome undergoes only slow loss of activity when stored at 4°C for up to six months.
If it is to be used on a regular basis, it is recommended that the material should be stored between 0°C-4°C during the duration of use.
For longer term storage the material may be dispensed as single use aliquots and stored at -80°C.

Handling

Avoid freeze/thaw cycles.

Scientific Background

The 20S proteasome is the catalytic core of the 26S proteasome, the central protease of the ubiquitin pathway of protein degradation.
Unlike the 26S proteasome, which degrades proteins in an ATP-dependent manner, the 20S proteasome is ATP-independent and only degrades entirely unfolded polypeptides.

 

Product Literature References
- The YΦ Motif Defines the Structure-Activity Relationships of Human 20S Proteasome Activators (Preprint): Kwadwo A. Opoku-Nsiah, Andres H. de la Pena, Sarah K. Williams, Nikita Chopra, Andrej Sali, Gabriel C. Lander, Jason E. Gestwicki, Abstract, Full Text

- Structural insights into substrate recognition and processing by the 20S proteasome: Sahu, MH Glickman - Biomolecules, 2021, Abstract

- Structural Insights into Substrate Recognition and Processing by the 20S Proteasome: Indrajit Sahu and Michael H. Glickman,  Faculty of Biology, Technion-Israel Institute of Technology, Biomolecules 2021, 11(2), 148, Abstract, Full Text